George S. Hussey, PhD

Assistant Professor of Pathology, Division of Experimental Pathology

Dr. Hussey is a member of the Division of Experimental Pathology.

Education & Training
BS - 2007, University of Akron, OH
PhD - 2012, Cleveland State University
Research Interests

My research interests are focused upon the discovery, development, and clinical translation of extracellular matrix (ECM)-based materials for functional tissue reconstruction. These interests also extend into an in-depth understanding of the molecular mechanisms of ECM-induced constructive remodeling, specifically in examining the remodeling response and the effect of matrix components on immune modulation toward a constructive, anti-inflammatory phenotype, with the broad goal of identifying molecular targets to bias these responses. Cell:Matrix interactions, including the role of matrix bound nanovesicles (MBV), constitutive phospholipids and incorporated (extracellular) RNA on matrix biology and the host tissue response, are prime targets of my investigations.

A second arm of my research is focused on the therapeutic potential of ECM hydrogels derived from decellularized tissues. The development of ECM hydrogels has greatly expanded the clinical utility of matrix scaffolds by offering minimally invasive delivery to sites of interest that are otherwise untenable for planar ECM sheets. A major focus of my research is to advance ECM hydrogel production from laboratory protocols to cGMP-compatible manufacturing processes that will overcome current challenges in ECM hydrogel production and enable clinical translation of this regenerative medicine-based therapy.

My future career research will continue to build upon the in-depth molecular characterization of ECM components with a strong translational focus toward the development of matrix-based therapeutic biomaterials. With a more thorough understanding of matrix composition, as well as the interplay between fibrillar and soluble components within matrix, we will be able to apply a more rational approach toward the design of transformative new technologies.

Representative Publications
  • Crum RJ, Capella-Monsonís H, Badylak SF, Hussey GS. Extracellular Vesicles for Regenerative Medicine Applications. Applied Sciences12(15), p.7472. (2022)
  • Hussey GS, Nascari DG, Saldin LT, Kolich B, Lee YC, Crum RJ, El-Mossier SO, D'Angelo W, Dziki JL, Badylak SF. Ultrasonic cavitation to prepare ECM hydrogels. (2020). Acta Biomaterialia. 108:77-86 (2020). PMID: 32268241.
  • Hussey GS, Pineda Molina C, Cramer MC, Tyurina Y, Tyurin VA, Lee YC, El-Mossier SO, Murdock MH, Timashev PS, Kagan VE, Badylak SF. Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials. Science Advances. 6(12):eaay4361.
  • Hussey GS, Dziki JL, Lee YC, Turnquist HR, Badylak SF. Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway. J Immunol Regen Med. 3:26-35 (2019). PMID: 31656879 (2020). PMID: 32219161.
  • Hussey GS, Keane TJ, Badylak SF. The Extracellular Matrix of the Gastrointestinal Tract: A Regenerative Medicine Platform. Nature Reviews Gastroenterology and Hepatology. 14(9):540-552 (2017). PMID: 28698662