Alejandro Soto-Gutierrez, MD, PhD

Professor of Pathology, Division of Experimental Pathology

Dr. Soto-Gutierrez is an member of the Division of Experimental Pathology and the Cellular and Molecular Pathology Graduate Training Program.

Research Interests

Clinical and Research Interests: Dr. Soto-Gutierrez’s clinical interest is focused on the treatment of liver diseases using regenerative and disruptive approaches with the goal of alleviating the currently growing problem of a scarcity of livers for transplantation. His research interest is focused on the development of new technologies for organ replacement using regenerative medicine approaches (auxiliary partial liver transplantation, cell transplantation, cell and organ engineering, genetic reprogramming) to re-generate entire replacement livers. His laboratory is actively working in liver cell differentiation and understanding hepatic maturation of Human induced Pluripotent Stem (iPS) cells using interactions with liver non-parenchymal liver cells, 3D liver matrix, and different molecules. While portions of the studies are carried out in organ culture systems, several ongoing studies are performed in specially-engineered animal models of liver regeneration to understand the repopulation capacities of hepatic tissue or liver grafts derived from human iPS cells.  In addition, Dr. Soto-Gutierrez’s laboratory is interested in strategies for reprogramming diseased livers and to induce super functions in livers (e.g. acute or chronic liver failure, liver steatosis, liver preservation). 

NIH Research

View Dr. Soto-Gutierrez's NIH RePORT on

Representative Publications

Peer-Reviewed Articles

View Dr. Soto-Gutierrez's publications on PubMed

  • Yagi H, Fukumitsu K, Fukuda K, Kitago M, Shinoda M, Obara H, Itano O, Kawachi S, Tanabe M, Coudriet GM, Piganelli JD, Gilbert TW, Soto-Gutierrez A*, Kitagawa Y. Human-Scale Whole-Organ Bioengineering for Liver Transplantation: a Regenerative Medicine Approach. Cell Transplant. 2012 *(Corresponding Author) (Accepted)
  • Liu L, Yannam GR, Nishikawa T, Yamamoto T, Basma H, Ito R, Nagaya M, Dutta-Moscato J, Stolz DB, Duan F, Kaestner KH, Vodovotz Y, Soto-Gutierrez A, Fox IJ. The microenvironment in hepatocyte regeneration and function in rats with advanced cirrhosis. Hepatology. 2012 May;55(5):1529-39.
  • Xu H, Berendsen T, Kim K, Soto-Gutierrez A, Bertheium F, Yarmush ML, Hertl M. Excorporeal normothermic machine perfusion resuscitates pig DCD livers with extended warm ischemia. J Surg Res. 2012 Apr;173(2):e83-8.
  • Shulman M, Cohen M, Soto-Gutierrez A, Yagi H, Wang H, Goldwasser J, Lee-Parsons CW, Benny-Ratsaby O, Yarmush ML, Nahmias Y. Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-Cyclodextrin. PLoS One. 2011 Apr 6;6(4):e18033. (In press)
  • Soto-Gutierrez A, Zhang L, Medberry CJ, Fukumitsu K, Faulk D, Jiang H, Reing JE, Gramignoli R, Komori J, Ross M, Nagaya M, Lagasse E, Beer Stolz D, Strom S, Fox IJ, Badylak SF. A Whole Organ Regenerative Medicine Approach for Liver Replacement. Tissue Eng Part C Methods. 2011 Mar 4. [Epub ahead of print]
  • Uygun K, Tolboom H, Izamis ML, Uygun B, Sharma N, Yagi H, Soto-Gutierrez A, Hertl M, Berthiaume F, Yarmush ML. Diluted blood reperfusion as a model for transplantation of ischemic rat livers: alanine aminotransferase is a direct indicator of viability. Transplant Proc. 2010 Sep;42(7):2463-7.
  • Soto-Gutierrez A, Yagi H, Navarro-Alvarez N, Nahmias Y, Goldwasser Y, Kitagawa Y, Tilles AW, Tompkins RG, Paekkadan B, Yarmush ML. Reactive Bone Marrow Stromal Cells Attenuate Systemic Inflammation via sTNFRI under the control of NFkB. Molecular Therapy. 2010, ct;18(10):1857-64.
  • Uygun B, Soto-Gutierrez A, Yagi H, Izamis ML, Shulman C, Milwid J, Kobayashi N, Nahmias Y, Tilles A, Berthiaume F, Hertl M, Yarmush ML, Uygun K. Organ Re-Engineering: Development of a Transplantable Recellularized Liver Graft using Decellularized Liver Matrix. Nat Medicine. 2010 2010 Jul;16(7):814-20.
  • Navarro-Alvarez N, Soto-Gutierrez A, Chen Y, Caballero-Corbalan J, Hassan W, Takei H, Iwamuro M, Yamamoto H, Tanaka N, Fox IJ and Kobayashi N. Intramuscular Transplantation of Engineered Hepatic Tissue Constructs Corrects Acute and Chronic Liver Failure in Mice. J Hepatol. 2010;52(2):211-9.
  • Navarro-Alvarez N, Kondo E, Kawamoto H, Hassan W, Yuasa T, Kubota Y, Seita M, Nakahara H, Hayashi T, Nishikawa Y, Hassan R, Javed SM, Noguchi H, Matsumoto S, Nakaji S, Tanaka N, Kobayashi N, Soto-Gutierrez A. Isolation and Propagation of a Human CD133-Negative Colon Tumor Derived Cell Line with Tumorigenic and Angiogenic Properties. Cell Transplant. 2010, 19(6):865-77.
  • Soto-Gutierrez A, Navarro-Alvarez N, Yagi H, Nahmias Y, Yarmush ML, Kobayashi N, Engineering of an Hepatic Organoide to Develop Liver Assist Devices. Cell Transplant. 2010, 19(6):815-22.
  • Soto-Gutierrez A, Yagi H, Kitagawa Y, Tilles AW, Tompkins RG, Yarmush ML. Bone Marrow Mesenchymal Stromal Cells Attenuate Organ Injury Induced by LPS and Burn . Cell Transplant. 2010, 19(6):823-30.
  • Van Poll D, Nahmias Y, Soto-Gutierrez A, Ghasemi M, Yagi H, Kobayashi N, Yarmush ML, Hertl M. Human Immune-reactivity against Liver Sinusoidal Endothelial Cells from GalTα(1,3)GalT-Deficient Pigs. Cell Transplant. 2010, 19(6):783-9.
  • Yamamoto T, Navarro-Alvarez N, Soto-Gutierrez A, Yuasa T, Iwamuro M, Kubota Y, Seita M, Kawamoto H, Javed SM, Kondo E, Noguchi H, Kobayashi S, Nakaji S, Kobayashi N. Treatment of acute liver failure in mice by hepatocyte xenotransplantation. Cell Transplant. 2010;19(6):799-806.
  • Yuasa T, Rivas-Carrillo JD, Navarro-Alvarez N, Soto-Gutierrez A, Kubota Y, Tabata Y, Okitsu T, Noguchi H, Matsumoto S, Nakaji S, Tanaka N, Kobayashi N. Neovascularization induced around an artificial device implanted in the abdomen by the use of gelatinized fibroblast growth factor 2. Cell Transplant. 2009;18(5):683-8.
  • Yagi H, Parekkadan B, Suganuma K, Soto-Gutierrez A, Tompkins R, Tilles A, Yarmush ML. Long Term Superior Performance of a Stem Cell/Hepatocyte Device for the Treatment of Acute Liver Failure. Tissue Engineering. 2009, Nov;15(11):3377-88.
  • Tolboom, H, Pouw, R, Izamis, ML, Milwid, JM, Sharma, N, Soto-Gutierrez, A, Nahmias, Y, Uygun, K, Berthiaume, F, Yarmush, ML. Recovery of Warm Ischemic Rat Liver Grafts by Normothermic Extracorporeal Perfusion. Transplantation 2009;87(2):170-7.


  • Soto-Gutierrez A*, Wertheim JA, Ott HC, Gilbert TW. Assembly of Organs for Clinical Use: Moving towards Transplantation on Demand. J Clin Invest. 2012 *(Corresponding Author) (Accepted)
  • Wertheim JA, Baptista PM, Soto-Gutierrez A*. Cellular therapy and bioartificial approaches to liver replacement. Curr Opin Organ Transplant. 2012 Apr 3. [Epub ahead of print] *(Corresponding Author)
  • Fukumitsu K, Yagi H, Soto-Gutierrez A*. Bioengineering in Organ Transplantation: Targeting the Liver. Transplant Proc. 2011;43(6):2137-8. *(Corresponding Author)
  • Soltys KA, Soto-Gutierrez A, Nagaya M, Baskin KM, Deutsch M, Ito R, Shneider BL, Squires R, Vockley J, Guha C, Roy-Chowdhury J, Strom SC, Platt JL, Fox IJ. Barriers to the successful treatment of liver disease by hepatocyte transplantation. J Hepatol. 2010 Oct;53(4):769-74.
  • Navarro-Alvarez N, Soto-Gutierrez A, Kobayashi N. Hepatic stem cells and liver development. Methods Mol Biol. 2010;640:181-236.
  • Soto-Gutierrez A, Yagi H, Uygun BE, Navarro-Alvarez N, Uygun K, Kobayashi N, Yang YG and Yarmush ML. Cell Delivery: From Cell Transplantation to Organ Engineering. Cell Transplant. 2010, 19(6):655-65.
  • Soto-Gutierrez A, Yagi H, Parekkadan B, Kitagawa Y, Tompkins RG, Kobayashi N, Yarmush ML. Mesenchymal Stem Cells: Mechanisms of Immunomodulation and Homing. Cell Transplant. 2010, 2010;19(6):655-65.
  • Yagi H, Tafaleng E, Nagaya M, Strom SC, Fox IJ, Soto-Gutierrez A. Embryonic and Induced Pluripotent Stem Cells as a Model for Liver Disease. Crit Rev Biomed Eng. 2009;37(4-5):377-98.